A glucanolytic Pseudomonas sp. associated with Smilax bona-nox L. displays strong activity against Phytophthora parasitica.
Identifieur interne : 000867 ( Main/Exploration ); précédent : 000866; suivant : 000868A glucanolytic Pseudomonas sp. associated with Smilax bona-nox L. displays strong activity against Phytophthora parasitica.
Auteurs : Ashraf S A. El-Sayed [Égypte] ; Asma Akbar [États-Unis] ; Irum Iqrar [États-Unis] ; Robina Ali [États-Unis] ; David Norman [États-Unis] ; Mary Brennan [États-Unis] ; Gul Shad Ali [États-Unis]Source :
- Microbiological research [ 1618-0623 ] ; 2018.
Descripteurs français
- KwdFr :
- ARN ribosomique 16S (génétique), Agents de lutte biologique (métabolisme), Facteur sigma (génétique), Maladies des plantes (parasitologie), Maladies des plantes (prévention et contrôle), Paroi cellulaire (métabolisme), Phytophthora (croissance et développement), Pseudomonas fluorescens (génétique), Pseudomonas fluorescens (isolement et purification), Pseudomonas fluorescens (métabolisme), Smilax (microbiologie).
- MESH :
- croissance et développement : Phytophthora.
- génétique : ARN ribosomique 16S, Facteur sigma, Pseudomonas fluorescens.
- isolement et purification : Pseudomonas fluorescens.
- microbiologie : Smilax.
- métabolisme : Agents de lutte biologique, Paroi cellulaire, Pseudomonas fluorescens.
- parasitologie : Maladies des plantes.
- prévention et contrôle : Maladies des plantes.
English descriptors
- KwdEn :
- Biological Control Agents (metabolism), Cell Wall (metabolism), Phytophthora (growth & development), Plant Diseases (parasitology), Plant Diseases (prevention & control), Pseudomonas fluorescens (genetics), Pseudomonas fluorescens (isolation & purification), Pseudomonas fluorescens (metabolism), RNA, Ribosomal, 16S (genetics), Sigma Factor (genetics), Smilax (microbiology).
- MESH :
- chemical , genetics : RNA, Ribosomal, 16S, Sigma Factor.
- chemical , metabolism : Biological Control Agents.
- genetics : Pseudomonas fluorescens.
- growth & development : Phytophthora.
- isolation & purification : Pseudomonas fluorescens.
- metabolism : Cell Wall, Pseudomonas fluorescens.
- microbiology : Smilax.
- parasitology : Plant Diseases.
- prevention & control : Plant Diseases.
Abstract
Biological control is an eco-friendly strategy for mitigating and controlling plant diseases with negligible effects on human health and environment. Biocontrol agents are mostly isolated from field crops, and microbiomes associated with wild native plants is underexplored. The main objective of this study was to characterize the bacterial isolates associated with Smilax bona-nox L, a successful wild plant with invasive growth habits. Forty morphologically distinct bacterial isolates were recovered from S. bona-nox. Based on 16S rRNA gene sequencing, these isolates belonged to 12 different genera namely Burkholderia, Pseudomonas, Xenophilus, Stenotrophomonas, Pantoea, Enterobactriaceae, Kosakonia, Microbacterium, Curtobacterium, Caulobacter, Lysinibacillus and Bacillus. Among them, Pseudomonas sp. EA6 and Pseudomonas sp. EA14 displayed the highest potential for inhibition of Phytophthora. Based on sequence analysis of rpoD gene, these isolates revealed a 97% identity with a Pseudomonas fluorescence strain. Bioactivity-driven assays for finding bioactive compounds revealed that crude proteins of Pseudomonas sp. EA6 inhibited mycelial growth of P. parasitica, whereas crude proteins of Pseudomonas sp. EA14 displayed negligible activity. Fractionation and enzymatic analyses revealed that the bioactivity of Pseudomonas sp. EA6 was mostly due to glucanolytic enzymes. Comparison of chromatographic profile and bioactivity assays indicated that the secreted glucanolytic enzymes consisted of β-1,3 and β-1,4 glucanases, which acted together in hydrolyzing Phytophthora cell walls. Since the biological activity of the crude glucanolytic extract was >60-fold higher than the purified β-1,3 glucanase, the glucanolytic enzyme system of Pseudomonas sp. EA6 likely acts synergistically in cell wall hydrolysis of P. parasitica.
DOI: 10.1016/j.micres.2017.11.018
PubMed: 29458848
Affiliations:
Links toward previous steps (curation, corpus...)
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El-Sayed</name><affiliation wicri:level="1"><nlm:affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA; Microbiology and Botany Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA; Microbiology and Botany Department, Faculty of Science, Zagazig University, Zagazig, 44519</wicri:regionArea><wicri:noRegion>44519</wicri:noRegion></affiliation></author><author><name sortKey="Akbar, Asma" sort="Akbar, Asma" uniqKey="Akbar A" first="Asma" last="Akbar">Asma Akbar</name><affiliation wicri:level="2"><nlm:affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Iqrar, Irum" sort="Iqrar, Irum" uniqKey="Iqrar I" first="Irum" last="Iqrar">Irum Iqrar</name><affiliation wicri:level="2"><nlm:affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Ali, Robina" sort="Ali, Robina" uniqKey="Ali R" first="Robina" last="Ali">Robina Ali</name><affiliation wicri:level="2"><nlm:affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Norman, David" sort="Norman, David" uniqKey="Norman D" first="David" last="Norman">David Norman</name><affiliation wicri:level="2"><nlm:affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Brennan, Mary" sort="Brennan, Mary" uniqKey="Brennan M" first="Mary" last="Brennan">Mary Brennan</name><affiliation wicri:level="2"><nlm:affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Ali, Gul Shad" sort="Ali, Gul Shad" uniqKey="Ali G" first="Gul Shad" last="Ali">Gul Shad Ali</name><affiliation wicri:level="2"><nlm:affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA. Electronic address: gsali@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author></analytic><series><title level="j">Microbiological research</title><idno type="eISSN">1618-0623</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological Control Agents (metabolism)</term><term>Cell Wall (metabolism)</term><term>Phytophthora (growth & development)</term><term>Plant Diseases (parasitology)</term><term>Plant Diseases (prevention & control)</term><term>Pseudomonas fluorescens (genetics)</term><term>Pseudomonas fluorescens (isolation & purification)</term><term>Pseudomonas fluorescens (metabolism)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Sigma Factor (genetics)</term><term>Smilax (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ribosomique 16S (génétique)</term><term>Agents de lutte biologique (métabolisme)</term><term>Facteur sigma (génétique)</term><term>Maladies des plantes (parasitologie)</term><term>Maladies des plantes (prévention et contrôle)</term><term>Paroi cellulaire (métabolisme)</term><term>Phytophthora (croissance et développement)</term><term>Pseudomonas fluorescens (génétique)</term><term>Pseudomonas fluorescens (isolement et purification)</term><term>Pseudomonas fluorescens (métabolisme)</term><term>Smilax (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Ribosomal, 16S</term><term>Sigma Factor</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Biological Control Agents</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN ribosomique 16S</term><term>Facteur sigma</term><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Smilax</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Smilax</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agents de lutte biologique</term><term>Paroi cellulaire</term><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="prévention et contrôle" xml:lang="fr"><term>Maladies des plantes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Biological control is an eco-friendly strategy for mitigating and controlling plant diseases with negligible effects on human health and environment. Biocontrol agents are mostly isolated from field crops, and microbiomes associated with wild native plants is underexplored. The main objective of this study was to characterize the bacterial isolates associated with Smilax bona-nox L, a successful wild plant with invasive growth habits. Forty morphologically distinct bacterial isolates were recovered from S. bona-nox. Based on 16S rRNA gene sequencing, these isolates belonged to 12 different genera namely Burkholderia, Pseudomonas, Xenophilus, Stenotrophomonas, Pantoea, Enterobactriaceae, Kosakonia, Microbacterium, Curtobacterium, Caulobacter, Lysinibacillus and Bacillus. Among them, Pseudomonas sp. EA6 and Pseudomonas sp. EA14 displayed the highest potential for inhibition of Phytophthora. Based on sequence analysis of rpoD gene, these isolates revealed a 97% identity with a Pseudomonas fluorescence strain. Bioactivity-driven assays for finding bioactive compounds revealed that crude proteins of Pseudomonas sp. EA6 inhibited mycelial growth of P. parasitica, whereas crude proteins of Pseudomonas sp. EA14 displayed negligible activity. Fractionation and enzymatic analyses revealed that the bioactivity of Pseudomonas sp. EA6 was mostly due to glucanolytic enzymes. Comparison of chromatographic profile and bioactivity assays indicated that the secreted glucanolytic enzymes consisted of β-1,3 and β-1,4 glucanases, which acted together in hydrolyzing Phytophthora cell walls. Since the biological activity of the crude glucanolytic extract was >60-fold higher than the purified β-1,3 glucanase, the glucanolytic enzyme system of Pseudomonas sp. EA6 likely acts synergistically in cell wall hydrolysis of P. parasitica.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29458848</PMID><DateCompleted><Year>2018</Year><Month>08</Month><Day>16</Day></DateCompleted><DateRevised><Year>2018</Year><Month>08</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-0623</ISSN><JournalIssue CitedMedium="Internet"><Volume>207</Volume><PubDate><Year>2018</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Microbiological research</Title><ISOAbbreviation>Microbiol Res</ISOAbbreviation></Journal><ArticleTitle>A glucanolytic Pseudomonas sp. associated with Smilax bona-nox L. displays strong activity against Phytophthora parasitica.</ArticleTitle><Pagination><MedlinePgn>140-152</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0944-5013(17)30011-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.micres.2017.11.018</ELocationID><Abstract><AbstractText>Biological control is an eco-friendly strategy for mitigating and controlling plant diseases with negligible effects on human health and environment. Biocontrol agents are mostly isolated from field crops, and microbiomes associated with wild native plants is underexplored. The main objective of this study was to characterize the bacterial isolates associated with Smilax bona-nox L, a successful wild plant with invasive growth habits. Forty morphologically distinct bacterial isolates were recovered from S. bona-nox. Based on 16S rRNA gene sequencing, these isolates belonged to 12 different genera namely Burkholderia, Pseudomonas, Xenophilus, Stenotrophomonas, Pantoea, Enterobactriaceae, Kosakonia, Microbacterium, Curtobacterium, Caulobacter, Lysinibacillus and Bacillus. Among them, Pseudomonas sp. EA6 and Pseudomonas sp. EA14 displayed the highest potential for inhibition of Phytophthora. Based on sequence analysis of rpoD gene, these isolates revealed a 97% identity with a Pseudomonas fluorescence strain. Bioactivity-driven assays for finding bioactive compounds revealed that crude proteins of Pseudomonas sp. EA6 inhibited mycelial growth of P. parasitica, whereas crude proteins of Pseudomonas sp. EA14 displayed negligible activity. Fractionation and enzymatic analyses revealed that the bioactivity of Pseudomonas sp. EA6 was mostly due to glucanolytic enzymes. Comparison of chromatographic profile and bioactivity assays indicated that the secreted glucanolytic enzymes consisted of β-1,3 and β-1,4 glucanases, which acted together in hydrolyzing Phytophthora cell walls. Since the biological activity of the crude glucanolytic extract was >60-fold higher than the purified β-1,3 glucanase, the glucanolytic enzyme system of Pseudomonas sp. EA6 likely acts synergistically in cell wall hydrolysis of P. parasitica.</AbstractText><CopyrightInformation>Copyright © 2017 The Authors. Published by Elsevier GmbH.. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>El-Sayed</LastName><ForeName>Ashraf S A</ForeName><Initials>ASA</Initials><AffiliationInfo><Affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA; Microbiology and Botany Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Akbar</LastName><ForeName>Asma</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Iqrar</LastName><ForeName>Irum</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ali</LastName><ForeName>Robina</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Norman</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brennan</LastName><ForeName>Mary</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ali</LastName><ForeName>Gul Shad</ForeName><Initials>GS</Initials><AffiliationInfo><Affiliation>Mid-Florida Research and Education Center, Department of Plant pathology, University of Florida/Institute of Food and Agricultural Sciences, Apopka, FL, USA. Electronic address: gsali@ufl.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>12</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Microbiol Res</MedlineTA><NlmUniqueID>9437794</NlmUniqueID><ISSNLinking>0944-5013</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D061046">Biological Control Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012808">Sigma Factor</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D061046" MajorTopicYN="N">Biological Control Agents</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011551" MajorTopicYN="N">Pseudomonas fluorescens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012808" MajorTopicYN="N">Sigma Factor</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032281" MajorTopicYN="N">Smilax</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biological activity</Keyword><Keyword MajorTopicYN="N">Glucanolytic system</Keyword><Keyword MajorTopicYN="N">Lipopeptides</Keyword><Keyword MajorTopicYN="N">Molecular identification</Keyword><Keyword MajorTopicYN="N">Pseudomonas spp.</Keyword><Keyword MajorTopicYN="N">Smilax bona-nox</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>05</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>11</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>11</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>2</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>2</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>8</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29458848</ArticleId><ArticleId IdType="pii">S0944-5013(17)30011-3</ArticleId><ArticleId IdType="doi">10.1016/j.micres.2017.11.018</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Égypte</li><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="Égypte"><noRegion><name sortKey="El Sayed, Ashraf S A" sort="El Sayed, Ashraf S A" uniqKey="El Sayed A" first="Ashraf S A" last="El-Sayed">Ashraf S A. El-Sayed</name></noRegion></country><country name="États-Unis"><region name="Floride"><name sortKey="Akbar, Asma" sort="Akbar, Asma" uniqKey="Akbar A" first="Asma" last="Akbar">Asma Akbar</name></region><name sortKey="Ali, Gul Shad" sort="Ali, Gul Shad" uniqKey="Ali G" first="Gul Shad" last="Ali">Gul Shad Ali</name><name sortKey="Ali, Robina" sort="Ali, Robina" uniqKey="Ali R" first="Robina" last="Ali">Robina Ali</name><name sortKey="Brennan, Mary" sort="Brennan, Mary" uniqKey="Brennan M" first="Mary" last="Brennan">Mary Brennan</name><name sortKey="Iqrar, Irum" sort="Iqrar, Irum" uniqKey="Iqrar I" first="Irum" last="Iqrar">Irum Iqrar</name><name sortKey="Norman, David" sort="Norman, David" uniqKey="Norman D" first="David" last="Norman">David Norman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= A glucanolytic Pseudomonas sp. associated with Smilax bona-nox L. displays strong activity against Phytophthora parasitica.
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